The present work deals with the isolation, purification and characterization of secondary metabolites of the fruits of Ziziphus mauritiana and the evaluation of their biological activity against Staphylococcus aureus. A plant from the Rhamnaceae family, widely used in the Cameroonian and Tchadian traditional pharmacopoeia for the treatment of various diseases such as food poisoning, pneumonia, urinary tract infections, and skin infections such as wounds and ulcers. Our investigations focused on the methanol/dichloromethane (MeOH/DCM, 1:1) extract of the fruits of Ziziphus mauritiana. Alkaloidal treatment was performed on this extract, and we obtained four (04) fractions: two non-alkaloid fractions, F1 (DCM), F2 (EtOAc), and two alkaloid fractions, F3 (DCM) and F4 (EtOAc). Using usual chromatographic methods (CC, CCM), we isolated from the alkaloidal fraction F3, two compounds 1 and 2, and four compounds 3, 4, 5, 6 from the non-alkaloid fraction F1, the hexane fraction. From these compounds, three (03) were fully characterized using spectroscopic methods 1H and 13C NMR (1 and 2 dimensions) and by comparison of their spectral data with those of the literature. These are: Sanjoinenine 1, betulinic acid 3, and epicatechin 4. The crude extract as well as fraction, and some isolated compounds were evaluated in vitro by the liquid microdilution method described by CLSI, against two strains of Staphylococcus aureus: S. aureus ATCC25923, S. aureus ATCC43300, and a Clinical isolate. The crude extract, fractions F1 and F4, were the most active fractions tested against the strain S. aureus ATCC 25923, with a MIC of 15.6, 62.5, and 15.6 µg/mL, respectively, while the other fractions (F2 and F3) showed moderate activity against the same strain. Fraction F2 is not active. For the S. aureus ATCC 43300, all the fractions have a weak activity, and for the clinical isolate, only ZSA shows a good activity with a MIC of 62.5 µg/mL, and the others show a moderate activity.
| Published in | American Journal of Applied Chemistry (Volume 14, Issue 1) |
| DOI | 10.11648/j.ajac.20261401.11 |
| Page(s) | 1-10 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2026. Published by Science Publishing Group |
Ziziphus Mauritiana, Staphylococcus Aureus, Sanjoinenine 1, Betulinic Acid 3, Epicatechin 4
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APA Style
Mahamat, A. A., Adannou, H. A., Heloua, H., Mbarga, P. E., Blandine, O. W., et al. (2026). Anti-Staphylococcus Aureus Guide Isolation of Compounds from the Fruits of Ziziphus Mauritiana. American Journal of Applied Chemistry, 14(1), 1-10. https://doi.org/10.11648/j.ajac.20261401.11
ACS Style
Mahamat, A. A.; Adannou, H. A.; Heloua, H.; Mbarga, P. E.; Blandine, O. W., et al. Anti-Staphylococcus Aureus Guide Isolation of Compounds from the Fruits of Ziziphus Mauritiana. Am. J. Appl. Chem. 2026, 14(1), 1-10. doi: 10.11648/j.ajac.20261401.11
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Download@article{10.11648/j.ajac.20261401.11,
author = {Aboubakar Ali Mahamat and Haroun Ali Adannou and Haroun Heloua and Paul Etoga Mbarga and Ouahouo Wache Blandine and Pierre Mkounga and Ephrem Augustin Nkengfack},
title = {Anti-Staphylococcus Aureus Guide Isolation of Compounds from the Fruits of Ziziphus Mauritiana},
journal = {American Journal of Applied Chemistry},
volume = {14},
number = {1},
pages = {1-10},
doi = {10.11648/j.ajac.20261401.11},
url = {https://doi.org/10.11648/j.ajac.20261401.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajac.20261401.11},
abstract = {The present work deals with the isolation, purification and characterization of secondary metabolites of the fruits of Ziziphus mauritiana and the evaluation of their biological activity against Staphylococcus aureus. A plant from the Rhamnaceae family, widely used in the Cameroonian and Tchadian traditional pharmacopoeia for the treatment of various diseases such as food poisoning, pneumonia, urinary tract infections, and skin infections such as wounds and ulcers. Our investigations focused on the methanol/dichloromethane (MeOH/DCM, 1:1) extract of the fruits of Ziziphus mauritiana. Alkaloidal treatment was performed on this extract, and we obtained four (04) fractions: two non-alkaloid fractions, F1 (DCM), F2 (EtOAc), and two alkaloid fractions, F3 (DCM) and F4 (EtOAc). Using usual chromatographic methods (CC, CCM), we isolated from the alkaloidal fraction F3, two compounds 1 and 2, and four compounds 3, 4, 5, 6 from the non-alkaloid fraction F1, the hexane fraction. From these compounds, three (03) were fully characterized using spectroscopic methods 1H and 13C NMR (1 and 2 dimensions) and by comparison of their spectral data with those of the literature. These are: Sanjoinenine 1, betulinic acid 3, and epicatechin 4. The crude extract as well as fraction, and some isolated compounds were evaluated in vitro by the liquid microdilution method described by CLSI, against two strains of Staphylococcus aureus: S. aureus ATCC25923, S. aureus ATCC43300, and a Clinical isolate. The crude extract, fractions F1 and F4, were the most active fractions tested against the strain S. aureus ATCC 25923, with a MIC of 15.6, 62.5, and 15.6 µg/mL, respectively, while the other fractions (F2 and F3) showed moderate activity against the same strain. Fraction F2 is not active. For the S. aureus ATCC 43300, all the fractions have a weak activity, and for the clinical isolate, only ZSA shows a good activity with a MIC of 62.5 µg/mL, and the others show a moderate activity.},
year = {2026}
}
TY - JOUR T1 - Anti-Staphylococcus Aureus Guide Isolation of Compounds from the Fruits of Ziziphus Mauritiana AU - Aboubakar Ali Mahamat AU - Haroun Ali Adannou AU - Haroun Heloua AU - Paul Etoga Mbarga AU - Ouahouo Wache Blandine AU - Pierre Mkounga AU - Ephrem Augustin Nkengfack Y1 - 2026/01/30 PY - 2026 N1 - https://doi.org/10.11648/j.ajac.20261401.11 DO - 10.11648/j.ajac.20261401.11 T2 - American Journal of Applied Chemistry JF - American Journal of Applied Chemistry JO - American Journal of Applied Chemistry SP - 1 EP - 10 PB - Science Publishing Group SN - 2330-8745 UR - https://doi.org/10.11648/j.ajac.20261401.11 AB - The present work deals with the isolation, purification and characterization of secondary metabolites of the fruits of Ziziphus mauritiana and the evaluation of their biological activity against Staphylococcus aureus. A plant from the Rhamnaceae family, widely used in the Cameroonian and Tchadian traditional pharmacopoeia for the treatment of various diseases such as food poisoning, pneumonia, urinary tract infections, and skin infections such as wounds and ulcers. Our investigations focused on the methanol/dichloromethane (MeOH/DCM, 1:1) extract of the fruits of Ziziphus mauritiana. Alkaloidal treatment was performed on this extract, and we obtained four (04) fractions: two non-alkaloid fractions, F1 (DCM), F2 (EtOAc), and two alkaloid fractions, F3 (DCM) and F4 (EtOAc). Using usual chromatographic methods (CC, CCM), we isolated from the alkaloidal fraction F3, two compounds 1 and 2, and four compounds 3, 4, 5, 6 from the non-alkaloid fraction F1, the hexane fraction. From these compounds, three (03) were fully characterized using spectroscopic methods 1H and 13C NMR (1 and 2 dimensions) and by comparison of their spectral data with those of the literature. These are: Sanjoinenine 1, betulinic acid 3, and epicatechin 4. The crude extract as well as fraction, and some isolated compounds were evaluated in vitro by the liquid microdilution method described by CLSI, against two strains of Staphylococcus aureus: S. aureus ATCC25923, S. aureus ATCC43300, and a Clinical isolate. The crude extract, fractions F1 and F4, were the most active fractions tested against the strain S. aureus ATCC 25923, with a MIC of 15.6, 62.5, and 15.6 µg/mL, respectively, while the other fractions (F2 and F3) showed moderate activity against the same strain. Fraction F2 is not active. For the S. aureus ATCC 43300, all the fractions have a weak activity, and for the clinical isolate, only ZSA shows a good activity with a MIC of 62.5 µg/mL, and the others show a moderate activity. VL - 14 IS - 1 ER -
Department of Organic Chemistry, University of Yaoundé I, Yaoundé, Cameroon
Department of Exact and Applied Sciences, Higher Teacher Training College of N'Djamena, N'djamena, Chad
